By Dr Desmond S. T. Nicholl
Des Nicholl provides a brand new, absolutely revised, and accelerated variation of his well known undergraduate-level textbook. The e-book keeps some of the gains of the unique version and nonetheless deals a concise technical advent to the topic of genetic engineering. it truly is divided into 3 major sections: simple molecular biology, tools of gene manipulation, and smooth purposes of genetic engineering. purposes lined within the ebook contain genomics, protein engineering, gene remedy, cloning, transgenic animals and crops, and bioethics. An creation to Genetic Engineering is vital studying for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.
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Extra info for An Introduction to Genetic Engineering (Studies in Biology)
Fractions can be collected and monitored for radioactivity, and the data used to calculate total activity of the DNA, speciﬁc activity, and percentage incorporation of the isotope. Radioactive labelling techniques are still used extensively in gene manipulation experiments, but pose some problems. There is the question of safety, as some high-energy radioisotopes are potentially hazardous. Thus the operator must be aware of the risks, and take appropriate precautions when using radioactive isotopes.
A) The 15 kb fragment yields two fragments of 14 and 1 kb when cut with BamHI. (b) The EcoRI fragments of 12 and 3 kb can be orientated in two ways with respect to the BamHI site. The BamHI/EcoRI double digest gives fragments of 11, 3 and 1 kb, and therefore the relative positions of the BamHI and EcoRI sites are as shown in (c). Similar reasoning with the orientation of the 8 and 7 kb PstI fragments (d) gives the ﬁnal map (e). on RNA. These may be required for many of the stages in the preparation and analysis of recombinants, but as they are not directly involved in the construction of recombinant DNA molecules, they will not be described in detail.
They carry multiple negative charges due to the phosphate groups on the phosphodiester backbone of the nucleic acid strands. This means that the molecules will migrate towards the positive electrode when placed in an electric ﬁeld. As the negative charges are distributed evenly along the DNA molecule, the charge/mass ratio is constant, thus mobility depends on fragment length. The technique is carried out using a gel matrix, which separates the nucleic acid molecules according to size. A typical nucleic acid electrophoresis setup is shown in Fig.
An Introduction to Genetic Engineering (Studies in Biology) by Dr Desmond S. T. Nicholl